Molecular biology of abiotic stress
Profile

Name: Surendra Chandra Sabat, Ph.D.

Date of Birth: 10th April, 1952

Mailing Address: Dr. S. C. Sabat, Plot # S-62, Maitree Vihar, Chandrasekharpur, Bhubaneswar-751023

Telephone: 0674-2301171 (H)
+91 9938866424 (M)

Retired Professor
(Scientist ‘G’ Grade)
Institute of Life Sciences, An Autonomous Organization Under Department of Biotechnology, Government of India, Bhubaneswar

Academics and service career

Master Degree in Botany: 1975, Berhampur University Odisha, Plant Science

Graduate degree (Ph. D.) 1977-1986, Kalyani University,
(Science) West Bengal
Biochemistry
Course work 1977-79

“Studies on the physiology of some aquatic vascular plants” (1986)

Supervisor Late Prof. Syamapada Saha

Post Doctoral Training: Bioenergetics  Laboratory
Late Prof. Prassana Mohanty
1986-1990
Jawaharlal Nehru University
Bioenergetics of
Photosynthesis.

Independent Scientific Programer

Assistant Professor: 1991, Institute of Life Sciences

Associate Professor: 1995, Institute of Life Sciences

Professor 2002, Institute of life Sciences

Scientist G 2007, Institute of Life Sciences

Superanuantion   2012

Post-superannuation appointments: 1. 2012-2014 (April)
Scientific Consultant to Institute of Life Sciences

2. Pre-Ph.D. Course co-ordinator

3. 2014 (March)
Adjunct Professor, Institute of Life Sciences.

Teaching experience:

As Guest Faculty to Department of Botany Utkal University

Bioenergetics of photosynthesis and respiration, Chlororespiration, Biophysical and Biochemical aspects of light energy utilization in photosynthesis, Functional modulation of thylakoid membrane structure and function to sustain abiotic stresses.

Protein structure and Function

Reactive oxygen metabolism in plant in response to light absorption and photosynthetic electron transport, Energy Budget, Plant’s metabolic adaptation
to varied ecological conditions.

Pre-Ph.D. Course work at Institute of Life Sciences

Light interaction with matter (Basics)

1. Absorption Spectroscopy
2. Fluorescence Spectroscopy (Excitation, Emission, Delayed Fluorescence, Anisotropy, FRIT)

Methodology (Principles of measurement)
Spectrophotometric and fluorimetric measurements, Spectral analysis (steady state, derivative, low temperature, CD)

Molecular biology of abiotic stress
Research activities:

Graduate level (1977-1984)

Major area of research activities was concentrated around the different aspects of photosynthesis to decipher the photosynthetic adaptation of submerged and floating aquatic angiospermic plants to their respective growth environments. The areas of research interest were carbon assimilation, phosphate esterification, photorespiratory activities and carboxylation reaction efficiencies.
Post-doctoral research:
During these period chloroplast photo-function was studied for deciphering the underlying mechanism of interaction of various electron transport and energy transfer inhibitors, electron donors and un-couplers. Further the senescence induced alternation in the chloroplast function was elaborately investigated.

As a independent researchers

Molecular studies on antioxidant enzymes and molecular recombinant approach to produce stress resistant enzyme protein and also raising transgenic crop plant with over expression of these enzymes
During this period the thrust area of research in my laboratory was to gain a better molecular understanding of the oxidative stress response and functioning of antioxidant enzymes in plants under stress conditions. During this period we could able to develop recombinant antioxidant proteins with site directed mutagenesis having light and temperature resistance. These mutated genes have been used to raise transgenic plants first with Arabidopsis to test the principle, and subsequently successfully transformed to rice plant.  We had also emphasized to decipher the functioning of dimeric and monomeric forms of CuZn-SOD in harboring the peroxidatic mode of function.

Molecular biology of abiotic stress
Publications as Independent Researcher (1991…..)

1. Tuteja N, Mishra P, Yadav S, Tajrishi M, Baral S and Sabat SC (2014) Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum. BMC Biotechnol. (In revision)
2. Mishra P , Dixit A , Ray M and Sabat SC (2014) Mechanistic study of CuZn-SOD from Ipomoea carnea mutated at dimer interface: enhancement of peroxidase activity upon monomerization. Biochimie, 97: 181-193.
3. Mishra P and Sabat SC (2013) A modified method of total RNA isolation and quantitative analysis of superoxide dismutase gene expression from different organs of Ipomoea carnea. Acta Biologica Szegediensis 57:121-129.
4. Bharati A, Kar M and Sabat SC (2012) Artemisinin inhibits chloroplast electron transport activity: mode of action. PLoS ONE 7(6): e38942. doi:10.1371/journal.pone.0038942.
5. Ray M, Mishra P , Das P and Sabat SC (2012) Expression and purification of soluble bio-active rice plant catalase-A from recombinant. J Biotech 157: 12-19.
6. Sahu GK and Sabat SC (2011) Changes in growth, pigment content and antioxidants in the root and leaf tissues of wheat plants under the influence of exogenous salicylic acid. Braz J Plant Physiol. 23: 209-218.
7. Bharati A and Sabat SC (2010) A spectrophotometric assay for quantification of artemisinin. Talanta 82: 1033-1037.
8. Sahu S, Das P, Ray M and Sabat SC (2010) Osmolyte modulated enhanced rice leaf catalase activity under salt-stress. Adv Biosci Biotech.1: 39-46.
9. Mondal P, Ray M, Sahu S and Sabat SC (2008) Co-expression of GroEL/ES enhances the expression of plant catalase in bacterial cytosol. Protein and peptide Letters 15: 1075-1078.
10. Mondal P. Ray M. Kar M, and Sabat SC (2008) Molecular Identification and properties of a light insensitive rice catalase-B expressed in E. coli. Biotechnol Lett. 30: 563-568.
11. Mondal P, Dey N, Dash AK, Chatterjee A, Sahu BB, Panda B, Maiti BI, and Sabat SC (2007) Structural and functional analysis of rice catalase-B promoter: presence of Dof and CAAT binding site. Plant Mol Biol Report (Online DOI 10.1007/S1105-007).
12. Sekhar PN, Kishor PBK, Reddy LA, Mondal P, Dash AK, Kar M, Mohanty S and Sabat SC (2006) In silico modeling and hydrogen peroxide binding study of rice catalase. In Silico Biol 6: 435-447.
13. Sahu GK, Kar M and Sabat SC (2002) Electron transport activities of isolated thylakoids from wheat plants grown in salicylic acid. Plant Biology 4: 321-328.
14. Sahu J and Sabat SC (2002) Iron superoxide dismutase in desiccation tolerant cyanobacteria. Ind J Microbiol. 42: 252-253.
15. Mishra SR and Sabat SC (1998) Effect of magnesium and calcium ion on the photoelectron transport activity of low slat suspended Hydrilla verticillata thylakoids; possible sites of cation interaction. Z. Naturforschung 53c: 849-856.
16. Mishra SR and Sabat SC (1998) Calcium and magnesium effect on divalent cation deficient Hydrilla verticillata thylakoid electron transport activity. J Biosci 23: 202-207.
17. Sabat SC, Padhye S and Mohanty P (1998) Inhibitory effects of synthetic lanthanum-crown at the reducing side of photosystem II. Z. Natuforsch 53 (c): 49-54.
18. Sabat SC (1996) Copper ion inhibition of electron transport activity in sodium chloride washed photosystem II particle is partially prevented by calcium ion. Z. Natuforsch 51(c): 179-184.
19. Mishra SR and Sabat SC (1995) Photosynthetic electron transport in Hydrilla verticillata (L.) is insensitive to methyl viologen (paraquat) inhibition. Biochem Biophys Res Commun 212: 132-137.
20. Mishra SR and Sabat SC (1995) Effect of calcium ion on Hydrilla verticillata thylakoid membrane oxygen evolution. Ind J Biochem Biophys. 32: 94-99.
21. Mishra SR and Sabat SC (1995) Characterization of ruthenium chloride supported Hill reaction in beet spinach thylakoids. Photosynthetica 31: 77-84.
22. Sabat SC and Mohanty P (1994) Photosynthetic adaptation in aquatic macrophytes. In: Purohit SS and Sahu MP edited Agro’s Annual Review of Plant Physiology (basic and Applied), Vol. 1 pp. 51-88, Agro Botanical publisher (India) Bikaner.

Post-Doctoral Publications

1. Sabat SC and Mohanty P (1992) Characterization of aliphatic amine 1,4-diazobicyclo (2,2,2) octane-induced stimulation of beet spinach thylakoid electron 
transport. Indian J Biochem Biophys. 29: 490-493.
2. Babu TS, Sabat SC and Mohanty P (1992) Alterations in photosystem II organization by cobalt treatment in the cyanobacterium Spirulina platensis. J Plant Biochem Biotech. 1:161-163.
3. Sabat SC, Babu TS and Mohanty P (1991) N, N-diethylhydroxylamine: a new electron donor to photosystem II. Biochem. Biophys Res Commun 179: 1127-1133.
4. Sabat SC, Murthy SDS and Mohanty P (1991) Differential thermal sensitivity of electron transport through photosystem 2 and 1 and proton up take in beet thylakoids. Photosynthetica 25: 75-80.
5. Sabat SC, Vijayavergiya V, Tripathy BC and Mohanty P (1991) Inhibitory effect of crown compounds on photoelectron transport activity of beet spinach thylakoid membranes. Z. Naturforsch 46c: 73-78.
6. Bansal KC, Sabat SC and Abrol YP (1991) Thylakoid Hill activity in relation to ploidy in wheat. Photosynthetica 25: 307-311.
7. Babu TS, Sabat SC and Mohanty P (1991) Heat induced alterations in the photosynthetic electron transport and emission properties of cyanobacterium Spirulina 
platensis. J Photochem Photobiol B 12: 161-171.
8. Sabat SC, Murthy SDS and Mohanty P (1991) Differential thermal sensitivity of electron transport through photosystems 2 and 1 and proton uptake in beet thylakoids. Photosynthetica 25: 75-80.
9. Bukhov NG, Sabat SC. and Mohanty P (1990) Analysis of chlorophyll a fluorescence changes in weak light in heat-treated Amaranthus chloroplasts. Photosynth. Res 23, 81-87.
10. Shubin VV and Sabat SC (1990) A simple room temperature spectrofluorimetric method for determination of chlorophyll a/b ratio. Indian J. Expt. Biol. 28: 87-90.
11. Murthy SDS, Sabat SC and Mohanty P (1989) Mercury-induced inhibition of photosystem II activity and changes in the emission of fluorescence from phycobilisomes in intact cells of the cyanobacterium, Spirulina platensis. Plant Cell Physiol. 30: 1153-1157.
12. Sabat SC, Grover A and Mohanty P (1989) Senescence induced alterations in the electron transport in wheat leaf chloroplast. J Photochem Photobiol, B: Biology. 3: 175-183.
13. Sabat SC, and Mohanty P (1989). Characterization of heat stress induced stimulation of photosynthetic electron transport activity in Amaranthus chloroplasts: effect of cations. J Plant Physiol. 133: 686-691.
14. Bukhov NG, Sabat SC and Mohanty P (1989) Sequential Loss of Photosynthetic Functions during Leaf desiccation as monitored by Chlorophyll Fluorescence Transient. Plant Cell Physiol. 30 393-398.
15. Grover A, Sabat SC and Mohanty P (1987) Does the loss of leaf chlorophyll during senescence of primary wheat leaf arise due to loss in chloroplast number or chlorophyll content? Biochem Physiol Pflanzen. 152: 481-484.
16. Sabat SC, Mohanty N and Mohanty P (1986) Heat induced alteration in electron donation site(s) of ascorbate and ascorbate-reduced catechol in the electron transport chain of Amaranthus chloroplasts. Ind J Biochem Biophys. 23: 266-269.
17. Grover A, Sabat SC and Mohanty P (1986) Relative sensitivity of various spectral forms of photosynthetic pigments to leaf senescence in wheat (Triticum aestivum). Photosynth Res. 10: 223-229.
18. Grover A, Sabat SC and Mohanty P (1986) Effect of temperature on photosynthetic activities of senescing detached wheat leaves. Plant Cell Physiol. 27: 117-126.

Graduate Study Publications

1. Sabat SC and Saha S (1986) Photosynthetic metabolism of dark fixed carbon in some aquatic angiosperms. Ind J Expt Biol 24: 517.
2. Sabat SC and Saha S (1998) Phosphate esterification in some aquatic angiosperms. Ind. J Exp Biol. 26: 360-362.

Full Publication in proceeding

1. Mohanty P, Sabat SC. and Padhye S (1991) Chemical probes for water oxidation complex in photosystem II. Reversible chloride anion pairing by K (picrate) (18-crown-6). Proceedings of the INDO-US Workshop on Impact of Global Climatic Changes of Photosynthesis and Plant Productivity, ed. Y.P. Abrol et al. (1991).
2. Sabat SC, Mohanty N, Hoshina S and Mohanty P. (1987) Effect of dimethylaminoethyl p-chlorophenoxyacetate (centrophenoxine) on chloroplast electron transport activitie. In: Progress in Photosynthesis Research. Ed. Biggins J. Vol.II , 581-584.

Book Chapter

1. Sabat SC, Grover A and Mohanty P (1989) Selective alterations in photosynthesis pigment chracteristic and photoelectron transport during senescence of wheat leaves. In: Singhal GS, Barber J, Dilley RA, Govindjee, Haselkorn R and Mohanty P (eds.) Photosynthesis – Molecular Biology and Bioenergetics, pp. 343-351. Narosa Publishing House, Delhi and Springer-Verlag, Berlin.

Indian Patent Filed: 

P. Mondal, M. Ray, S. Sahu, and S. C. Sabat – Patent Title “A genetically modified recombinant rice plant catalase-B, expressed in E. coli host that avoids light and temperature impairment”. No.1180/KOL/07 of 27.08.07.

NCBI Submissions
DQ078758 Oryza sativa (Indica cultivar-group) catalase-B mRNA Complete cds
DQ118681 Oryza sativa (Indica cultivar-group) catalase-C, mRNA, complete cds
EF371902 Oryza sativa (Indica cultivar-group) catalase (CatA) mRNA, complete cds
DQ082895 RAPD fragment from submerged leaves of Potamogeton nodosus with dof signature
EF182719 Oryza sativa (Indica cultivar-group) catalase (CatB) gene, partial cds

Molecular biology of abiotic stress
Ph. D. Thesis Supervised

Awardee Title

1. Dr. Sujata Rani Mishra Studies on thylakoid photo-function of a
   (Botany, ILS) submerged aquatic angiosperm. Hydrilla verticillata (1998).
2. Dr. Guru Charan Nayak Changes in antioxidant system in germinating
   (Botany, Utkal University) and callus forming rice seeds (2002)
3. Dr. Gopal Krishna Sahu Studies on some physiological processes in (Botany, ILS) wheat (Triticum aestivum Var. Sonalika);
   Effect of salicylic acid (2004)
4. Dr. Ardhendu Kumar Dash Catalase activity in germinating rice embryo (Botany, Utkal University) (2006)
5. Dr. Prosenjit Mondal Characterization of catalase gene and catalase (Life Science, ILS) gene product in rice (2008)
6. Dr. (Mrs.) Mamata Ray Molecular and biochemical characterization
   (Biotechnology, ILS) and expression analysis of a gene encoding catalase in Oryza sativa (2011)
7. Dr. Priyanka Das Characterization of rice catalase- B and
   (Botany, Utkal University) catalase-B gene mutant products in transgenic Arabidopsis (2011)
8. Dr. (Mrs.) Adyasha Bharati Phytotoxic effect of artemisinin on the
   (Environmental Science) photosynthetic activity of rice leaves (2013)
   Utkal University

Submitted

Mr. Panchanad Mishra Molecular and Biochemical characterization
(Life Science, ILS) of a recombinantly expressed Cu-Zn superoxide dismutase from Ipomoea carnea (2014).

Research assistants:

Mr. Sudhir Baral Raising transgenic rice with over expression of rice Catalase-B in its normal and mutated form

Ms. Rashmi Satapathy Molecular interaction of artemisinin with heme containing proteins and hemoglobin

Molecular biology of abiotic stress